Anti-CD74 Antibody

The CD74 antigen, also known as the MHC class II invariant chain (Ii), plays a central role in antigen presentation and chaperones MHC class II molecules through the endosomal pathway. This unconjugated rabbit polyclonal reagent from Antibodies.com targets human CD74 and is validated for Western blot and immunohistochemistry workflows.

The antibody recognizes the type II transmembrane glycoprotein expressed on B lymphocytes, monocytes, macrophages, and dendritic cells, as well as certain epithelial and thymic epithelial populations. Beyond its role in MHC class II trafficking, CD74 functions as a cell-surface receptor for macrophage migration inhibitory factor (MIF) and its homolog D-DT, linking it to signaling cascades that influence B-cell maturation, survival, and inflammatory responses.

Each vial is supplied as 100 µl of unconjugated antiserum, enabling flexibility in downstream detection chemistries including HRP, biotin, or fluorophore conjugates selected by the end user. The polyclonal format offers recognition of multiple epitopes on the CD74 protein, which can be advantageous when probing denatured targets in Western blot or formalin-fixed paraffin-embedded tissue sections in IHC.

Researchers studying autoimmune disease, antigen processing, lymphoid neoplasms, and MIF-driven inflammatory signaling pathways commonly apply this reagent. The host species is rabbit, clonality is polyclonal, and the antibody is intended for research use only. To preserve activity, aliquot upon receipt and minimize freeze-thaw cycles during routine use.