Anti-CD74 Antibody

The CD74 invariant chain (Ii) plays a central role in MHC class II assembly and antigen presentation, acting as a chaperone that guides the αβ heterodimer through the endoplasmic reticulum and endosomal compartments where CLIP is exchanged for antigenic peptide. Antibodies.com supplies this unconjugated rabbit polyclonal reagent for western blot and immunohistochemistry workflows, enabling researchers to probe CD74 expression in human B cells, dendritic cells, macrophages, and MHC class II-positive epithelial tissues. The antibody is delivered in a 100 µl vial, sufficient for multiple immunoblot and tissue section staining runs. Typical laboratory applications include characterization of B-cell maturation stages, profiling of lymphoma and chronic lymphocytic leukemia biopsies, validation of CD74 knockdown or overexpression in cell culture models, and investigation of macrophage migration inhibitory factor (MIF) receptor signaling. For long-term use, aliquot the reagent on arrival and store aliquots frozen, avoiding repeated freeze-thaw cycles to preserve polyclonal recognition of epitopes across the cytoplasmic and luminal domains of the invariant chain. The product is intended for in vitro research use and is reactive with human samples only.