Anti-CD73 Antibody

Manufactured by Antibodies.com, this research-grade primary antibody is a rabbit polyclonal reagent raised against CD73 (ecto-5'-nucleotidase, NT5E), a GPI-anchored ectoenzyme involved in purinergic signalling and adenosine generation. Supplied as a 50 µl liquid aliquot at a working concentration of 1 mg/ml, the antibody is unconjugated, allowing it to be paired freely with secondary detection chemistries of the researcher's choice. The host species is rabbit and the clonality is polyclonal, providing a broad epitope recognition profile useful for capturing conformational and linear determinants of the target. The reagent is validated for Western Blot (WB) and ELISA applications and has been confirmed to react with human samples, making it suitable for studies of CD73 expression in human cell lines, primary tissues, and lysates. The product is certified for Research Use Only and is classified as a primary antibody within the Antibodies.com catalogue, intended for laboratory investigation rather than diagnostic or therapeutic use. Recommended storage is short term at 4 °C for routine handling, with long-term storage at -20 °C in aliquots to avoid repeated freeze/thaw cycles; the buffer formulation is PBS-based with stabilisers. Researchers working on cancer immunology, tumour microenvironment biology, T-cell regulation, or inflammation pathways where CD73 activity modulates immunosuppressive adenosine signalling will find this polyclonal reagent a dependable tool for both qualitative detection by WB and quantitative measurement by ELISA. Each lot is quality-controlled by the supplier to ensure batch-to-batch consistency, and the antibody integrates seamlessly into standard immunodetection workflows when paired with appropriate HRP- or fluorophore-conjugated anti-rabbit IgG secondary antibodies.