Anti-CD69 Antibody

The CD69 antigen, a C-type lectin family member encoded at the CD69 locus (also referenced as CLEC2C), functions as a rapidly induced early activation marker on T cells, B lymphocytes, and natural killer cells. Surface expression rises within hours of mitogenic or antigenic stimulation, peaking around 18 to 24 hours, which makes CD69 a widely used read-out in immunology research for tracking lymphocyte activation kinetics in vitro.

This unconjugated rabbit polyclonal reagent was raised against the human CD69 target and is validated for Western blot and ELISA workflows across human, mouse, and rat samples. The polyclonal format from Antibodies.com provides broad epitope coverage, supporting detection of the mature glycosylated antigen in cell lysates and tissue-derived preparations. The reagent is supplied as a 100 µl liquid aliquot in a single-use vial format suitable for direct dilution into standard assay buffers.

Investigators commonly apply this antibody to profile early T-cell activation in cell culture systems, characterize NK-cell degranulation responses, and study signal-transduction events downstream of T-cell receptor engagement. Unconjugated format leaves the choice of reporter open: the reagent can be paired with HRP-, biotin-, or fluorophore-conjugated secondary antibodies tailored to the experimental readout. For long-term storage, refrigerate the vial; for working aliquots, freeze at lower temperatures and limit repeated freeze-thaw cycles to preserve binding activity.