Anti-CD5L Antibody

This Rabbit polyclonal antibody targets CD5L (CD5 antigen-like), a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) family that is expressed abundantly by tissue-resident macrophages, including Kupffer cells in the liver, and circulates in serum. Antibodies.com provides this reagent as an unconjugated IgG preparation supplied in a 200 µl vial, validated for Western blot detection of the human and mouse target. CD5L — also called Spα or AIM — has been studied in the contexts of inflammation, lipid handling by macrophages, control of bacterial infection, and hepatocellular stress responses, where researchers use Western blotting to track its expression in cell lysates from primary macrophages, macrophage cell lines, and liver tissue. The polyclonal format offers multiple epitope coverage, useful when probing a single band in whole-cell extracts or when follow-up validation against recombinant CD5L is planned. For sample preparation, typical Western blot workflows load 20–40 µg of total protein per lane under reducing conditions, with the antibody commonly applied at 0.5–2 µg/mL after transfer to nitrocellulose or PVDF. Refrigerate the vial for short-term use and aliquot for repeated experiments to limit freeze-thaw cycles; the IgG fraction remains stable under these conditions. Shipping is generally on blue ice to preserve antibody integrity, and the unconjugated format allows pairing with HRP- or fluorescent-labeled secondary reagents of the researcher's choice. Reactivity spans both human and mouse samples, supporting cross-species comparative studies of macrophage biology, including work on atherosclerosis models, liver injury, and innate immune signaling.