Anti-CD54 (ICAM-1) Antibody

The mouse monoclonal antibody from Antibodies.com targets human CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1), a cell surface immunoglobulin superfamily glycoprotein that mediates adhesion between leukocytes and the vascular endothelium during inflammatory responses. Supplied as an unconjugated IgG2b reagent in a 100 µl volume, this primary antibody is validated for Western blot (WB) and immunocytochemistry (ICC) workflows and is reactive with human ICAM-1.

ICAM-1 is constitutively expressed at low levels on endothelial cells, epithelial cells, and fibroblasts, and is strongly upregulated by pro-inflammatory cytokines such as TNF-α, IL-1β, and IFN-γ, making it a widely used marker for studying endothelial activation, leukocyte transmigration, and vascular inflammation in immunology and cell biology research.

Key features include monoclonal specificity for reduced batch-to-batch variability, an IgG2b isotype compatible with standard secondary antibody detection systems, and an unconjugated format allowing flexible pairing with fluorophore or enzyme labels of the researcher's choice. The reagent should be stored refrigerated and aliquoted to limit freeze-thaw cycles across multiple experiments. Researchers investigating immune cell trafficking, atherosclerosis, transplant rejection, or chronic inflammatory disease mechanisms can apply this antibody to probe ICAM-1 expression patterns in cultured human cells and tissue preparations.