Anti-CD47 Antibody

CD47 is a ubiquitously expressed transmembrane glycoprotein recognized for its role as a 'don't eat me' checkpoint signal through interaction with SIRPα on macrophages, with extensive use in cancer immunology, cell signaling, and transplantation research. This unconjugated rabbit polyclonal reagent from Antibodies.com targets the CD47 antigen and is supplied as a 100 µl vial, validated for western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat tissue or cell samples. The polyclonal format offers multi-epitope recognition of the target protein. Researchers commonly pair this reagent with appropriate HRP- or fluorophore-conjugated secondary antibodies to amplify signal in chromogenic IHC or immunofluorescence imaging. The antibody is used in in vitro studies examining CD47-SIRPα signaling pathways, tumor immune evasion mechanisms, and comparative analysis of CD47 expression across species, making it a versatile tool for both basic research and translational investigations of immune checkpoint biology.