Anti-CD44 Antibody

This mouse monoclonal antibody targets the human CD44 transmembrane glycoprotein and is supplied by Antibodies.com as an unconjugated primary reagent for research workflows. CD44 functions as the principal cell-surface receptor for hyaluronic acid and participates in lymphocyte homing, hematopoiesis, and the rolling interaction of leukocytes on vascular endothelium. The reagent is formulated in 1×TBS at pH 7.4 with 1% BSA and 40% glycerol as a cryoprotectant, with 0.05% sodium azide added as a preservative, and is provided in a 100 µl vial.

Validated applications include Western blot, immunohistochemistry, and flow cytometry, supporting detection of CD44 across denatured protein lysates, fixed tissue sections, and live-cell surface staining. The antibody was raised in mouse and is of the IgM isotype, and it has been characterized for reactivity with human CD44, supporting research into tumor metastasis, stem cell markers, and immune cell migration where CD44v isoforms are commonly investigated. Store refrigerated and protected from light; for long-term storage the glycerol content permits freezing at -20 °C in working aliquots to limit repeated freeze-thaw cycles.

Researchers using this reagent in flow cytometry can pair it with a labeled anti-mouse IgM secondary antibody, while immunohistochemistry and Western blot workflows can be set up with standard detection protocols optimized for IgM primaries. The product is shipped by Antibodies.com as 100 µl of ready-to-dilute liquid, and the formulation is compatible with most common staining, blotting, and assay buffers.