Anti-CD40 Ligand Antibody

Raised in rabbit against the human CD40 Ligand (CD154) costimulatory protein, this polyclonal antibody from Antibodies.com is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a 50 µl vial suited to western blot, immunohistochemistry, and immunofluorescence workflows. CD40 Ligand is a type II transmembrane member of the TNF superfamily expressed on activated CD4+ T cells, platelets, and other immune populations, where engagement of CD40 on B cells, macrophages, and dendritic cells drives immunoglobulin class switching, germinal center formation, and cytokine release. Researchers apply this reagent to map CD40L distribution in human tissue sections, track activation-induced upregulation on T cell cultures, and probe receptor signaling in models of autoimmunity, transplant rejection, and atherosclerosis. The unconjugated format permits flexible pairing with custom fluorophores or HRP reporters depending on the chosen application. For handling, the glycerol-containing buffer resists freeze-thaw cycles; small aliquots should be refrigerated for routine use, and the stock is best stored frozen at -20°C between sessions to preserve the polyclonal response against the extracellular and intracellular epitopes of CD40 Ligand.