Anti-CD40 Antibody [5C3]

The 5C3 clone from Antibodies.com targets the human CD40 antigen, a 48 kDa transmembrane glycoprotein belonging to the TNF receptor superfamily. CD40 is expressed on B lymphocytes, dendritic cells, macrophages, and additional antigen-presenting cells, where it mediates costimulatory signaling essential for B cell activation and adaptive immune responses. This unconjugated IgG1 reagent is supplied as a 100 ug liquid preparation and is validated for western blot, immunocytochemistry, flow cytometry, and immunohistochemistry workflows. Researchers routinely apply the 5C3 reagent for immune cell phenotyping, mapping CD40–CD154 (CD40L) interactions, and characterizing B cell subsets in lymphoma and autoimmune studies. For storage, keep the vial at -20 °C in single-use aliquots to minimize freeze-thaw cycles, protect from light, and use for research purposes only.