Anti-CD3G Antibody

CD3G, the gamma chain component of the T-cell receptor-CD3 complex, sits on the surface of mature T lymphocytes and plays an essential role in TCR signaling and T-cell development. The Antibodies.com polyclonal anti-CD3G reagent is raised in rabbit and supplied unconjugated, recognizing the human, mouse, and rat forms of the target to support comparative immunological work across rodent disease models, primary human tissue samples, and cultured T-cell lines.

The antibody is formulated at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2. Each vial delivers 100 µl of ready-to-use stock, providing ample volume for dozens of membrane-based detection runs when used at standard working concentrations. For Western blot applications, the reagent is commonly paired with HRP-conjugated secondary antibodies and detected via chemiluminescence, allowing clean separation of the gamma chain band from associated CD3 subunits.

Research applications extend into studies of T-cell maturation, receptor signaling cascades, primary immune deficiency, and characterization of immune-cell infiltrates within tumor and inflammatory tissue samples. Short-term storage at 4 °C is appropriate for active vials, with aliquots held at -20 °C for long-term stability; repeated freeze-thaw cycles should be avoided and the vial protected from light. This is an in vitro reagent intended for research use only, and researchers should observe standard precautions when handling the sodium azide-containing formulation.