Anti-CD31/PECAM-1 Antibody

Recombinant monoclonal reagent from Antibodies.com targets the CD31/PECAM-1 antigen, a 130 kDa transmembrane glycoprotein of the immunoglobulin superfamily most densely expressed on vascular endothelial cells, platelets, and select leukocyte subsets. The unconjugated antibody is supplied as 50 µl of rabbit monoclonal antibody in TBS buffer (pH 7.4) supplemented with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative.

Validated for western blot, immunohistochemistry, and flow cytometry on human samples, this reagent serves as a workhorse marker in studies of angiogenesis, vascular integrity, and tumor microvasculature characterization. Researchers use it to delineate capillary networks in fixed tissue sections, to confirm endothelial lineage in cultured cell populations, and to investigate leukocyte transmigration events where PECAM-1 homophilic interactions drive diapedesis across the vessel wall. Its presence on platelets also supports investigations into thrombotic signaling and megakaryocyte biology.

The rabbit host and recombinant monoclonal construction provide consistent specificity, reducing background staining in multiplex immunohistochemistry panels. The unconjugated format allows researchers to couple the antibody to fluorophores, biotin, or enzymes for custom assay development. For storage, refrigerate the vial short term and aliquot before freezing for extended preservation, protecting the solution from repeated freeze-thaw cycles to maintain antigen-binding activity. Each preparation is quality-controlled by Antibodies.com for the listed applications and is intended for research use only.