Anti-CD3-ε Antibody

The CD3-ε chain is a transmembrane signaling subunit of the T-cell receptor (TCR) complex, essential for surface expression of the TCR and for coupling antigen recognition to intracellular activation cascades in T lymphocytes. This polyclonal reagent from Antibodies.com targets the epsilon subunit specifically, enabling selective detection of CD3-ε in human samples through Western blot and ELISA workflows.

The unconjugated format allows researchers to couple the antibody to their preferred detection label depending on the assay, and the polyclonal nature provides recognition of multiple epitopes on the epsilon chain, which can be advantageous when probing for protein variants or denatured targets. The reagent is supplied as 100 µl of polyclonal antiserum raised in rabbit and is validated for reactivity with human CD3-ε, making it relevant for studies of T-cell development, TCR signaling, thymic selection, and immune phenotyping in human cell and tissue samples.

Applications include characterization of T-cell populations, investigation of immune checkpoint pathways, and analysis of CD3 expression in primary lymphocytes and established T-cell lines. The reagent can be applied to whole-cell lysates, membrane fractions, or purified TCR preparations in Western blot, and to immobilized antigen in ELISA, supporting both qualitative and quantitative readouts of CD3-ε levels in research samples.