Anti-CD3-ε Antibody

CD3-ε is a signaling subunit of the T-cell receptor complex, and antibodies targeting this protein are widely used to identify T lymphocytes in tissue sections and cell preparations. This rabbit polyclonal reagent from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide, packaged as a 100 µl vial of unconjugated antibody. It has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples, supporting studies of T-cell development, thymic selection, and immune signaling. Researchers commonly apply it to investigate T-cell infiltration in tumor microenvironment studies, characterize lymphoid organ architecture, and probe signaling pathways downstream of T-cell receptor engagement. The polyclonal format increases the likelihood of recognizing multiple epitopes on the epsilon chain, which can be helpful when working with denatured samples for Western blot or with fixed tissue for IHC. Store refrigerated and protect from light to preserve activity; aliquot if repeated freeze-thaw cycles are anticipated.