Anti-CD298 Antibody

CD298, the broadly expressed beta-3 subunit of the human Na+/K+-ATPase (ATP1B3), is a type II transmembrane glycoprotein found on the plasma membrane of virtually all nucleated cells. This rabbit polyclonal reagent from antibodies.com binds the human target and is supplied as 100 µl of unconjugated antisera, validated for Western blot and ELISA on human samples.

Because CD298 is constitutive on most cell types, the antibody is often deployed as a membrane-protein loading reference alongside lineage markers such as CD45 in flow cytometry panels, or used to normalize surface-protein input across lanes in immunoblotting. In SDS-PAGE the beta-3 subunit resolves in the 40-55 kDa region, producing a defined band suitable for densitometric quantification. The polyclonal format broadens epitope coverage, while the unconjugated state leaves the choice of secondary reporter open for HRP, alkaline phosphatase, or fluorophore-based detection systems.

Storage: aliquot the stock vial upon arrival and store at -20 °C in working volumes to limit freeze-thaw cycles. Short-term handling at 4 °C is acceptable for active aliquots; avoid prolonged room-temperature exposure of the stock. For research use only.