Anti-CD27 Antibody - Identical to Abcam (ab175403)

From Antibodies.com, this rabbit polyclonal IgG antibody targets CD27 and is supplied as an unconjugated primary antibody reagent. It was raised against a recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1) and is validated for Western blot, immunohistochemistry, and immunocytochemistry/immunofluorescence in Human, Mouse, and Rat samples.

The antibody is affinity purified and supplied as a liquid in Phosphate Buffered Saline (pH 7.3) with 50% glycerol and 0.01% Thiomersal, with a reported molecular weight of 55 kDa. Recommended working dilutions are 1:500-1:1,000 for WB, 1:50-1:200 for IHC, and 1:50-1:200 for ICC/IF. Concentration is reported as lot specific.

For storage, the reagent is shipped at 4°C; upon delivery it should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided.