Anti-CD22 Antibody

Produced by Antibodies.com, this rabbit polyclonal reagent targets CD22, a B-cell lineage marker belonging to the Siglec family of sialic acid-binding lectins. The product is supplied as a 100 µl vial at 1 mg/ml in PBS buffer containing 0.1% Sodium Azide and 50% glycerol, allowing straightforward use in Western blot and immunohistochemistry workflows.

The antibody recognizes CD22, a transmembrane glycoprotein predominantly expressed on mature B lymphocytes where it modulates B-cell receptor signaling through ITIM-mediated recruitment of phosphatases. Cross-reactivity studies demonstrate specific binding to both human and mouse CD22, supporting its use across species in lymphoma research, autoimmune disease models, and immune cell phenotyping experiments.

For storage, the antibody should be kept at -20°C in small aliquots to minimize freeze-thaw cycles and preserve affinity. The polyclonal format provides epitope diversity that supports detection of both native and denatured CD22 across nitrocellulose membranes and formalin-fixed paraffin-embedded tissue sections. Sodium azide in the formulation inhibits microbial contamination, making the vial stable for repeated use during extended experimental campaigns.