Anti-CD1A Antibody

CD1A is a transmembrane MHC class I-like glycoprotein that presents lipid and glycolipid antigens to T cells, and is widely used as a marker for cortical thymocytes and Langerhans cells in immunology and pathology research. This unconjugated rabbit polyclonal reagent from Antibodies.com enables detection of the human CD1A protein in both denatured lysate preparations and fixed tissue sections.

Each vial contains 100 µl of antiserum with a polyclonal clonality that recognizes multiple epitopes across the CD1A molecule, supporting consistent signal in western blot analysis and clear visualization of CD1A-positive dendritic cells by immunohistochemistry. The antibody reacts specifically with human CD1A and supports studies of antigen presentation, thymic development, and the immunophenotyping of Langerhans cell histiocytosis lesions.

For western blot, run lysates from CD1A-expressing cell lines or primary human dendritic cells under standard reducing conditions and probe at a working dilution appropriate for the detection system in use. For immunohistochemistry, the reagent can be applied to paraffin-embedded or frozen sections of skin, thymus, and lymphoid tissue to highlight Langerhans cells and related CD1A-bearing populations. Store aliquots at -20°C and protect from light to preserve antibody activity across multiple freeze-thaw cycles.