Anti-CD177 Antibody

This unconjugated Rabbit polyclonal reagent from Antibodies.com targets CD177, a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein expressed almost exclusively on a subset of human neutrophils, where it is also known as NB1, PRV-1, and the alloantigen HNA-2a. The antibody is supplied as 100 µl of polyclonal antiserum for western blot and ELISA workflows, with reactivity validated against human samples.

Researchers use CD177 as a marker of neutrophil activation and transendothelial migration, since the protein mediates adhesion through interactions with PECAM-1 (CD31) on endothelial cells. Upregulation of CD177 mRNA and surface expression is well documented in myeloproliferative neoplasms such as polycythemia vera, making this reagent useful in studies of neutrophil biology, inflammatory disease, and diagnostic marker panels.

For western blot applications, the primary antibody is commonly applied at 1:500 to 1:2000 dilutions depending on antigen abundance, and the polyclonal format allows recognition of multiple epitopes across the mature glycoprotein. Aliquot upon receipt to limit freeze-thaw cycles and preserve antibody activity across repeated experiments.