Anti-CD133 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets CD133, a transmembrane glycoprotein also known as Prominin-1 that is routinely used to identify and isolate cancer stem cell populations, hematopoietic progenitors, and neural stem cells. Supplied as 100 µl of ready-to-use reagent, it supports both Western blotting and ELISA workflows against human and mouse samples.

The CD133 antigen bears five transmembrane domains and localizes to cellular protrusions, and it is frequently cited in studies of glioblastoma, colorectal carcinoma, hepatocellular carcinoma, and other tumour types where CD133-positive subpopulations exhibit self-renewal capacity and tumourigenic potential. Researchers investigating regenerative biology rely on this marker to track stem and progenitor cells during differentiation protocols, while cancer researchers use it to enrich stem-like fractions for drug-resistance and xenograft assays.

Each lot is validated for reactivity across both human and mouse systems, giving laboratories a single reagent for cross-species studies of stem cell markers, tumour-initiating cells, and developmental biology. The polyclonal format offers epitope diversity across the CD133 molecule, which is useful when working with samples where expression levels vary or where post-translational modifications may obscure individual epitopes on a monoclonal target.

Applications include immunoblotting of membrane-enriched fractions and ELISA-based quantitation, both of which benefit from the unconjugated format that allows downstream labelling with HRP, biotin, or fluorophore conjugates chosen by the end user.