Anti-CD101 Antibody

This rabbit polyclonal antibody targets CD101, a transmembrane immunoglobulin superfamily member expressed on activated T lymphocytes, monocytes, granulocytes, and certain dendritic cell subsets. Raised in rabbit and affinity-purified, the reagent is supplied unconjugated at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.3. Validated for Western blot, the antibody recognizes denatured CD101 from human, mouse, and rat samples, supporting comparative studies of leukocyte activation, regulatory T cell biology, and inflammatory signalling pathways.

For routine work, store the 100 µl vial refrigerated short term; for longer storage, aliquot into single-use portions and freeze at -20 °C to limit freeze-thaw cycles. Protect from light because the azide-containing buffer is light-sensitive. Sodium azide is incompatible with live cell assays, so researchers should dialyze or gel-filter the preparation before live-cell or in vivo applications. The polyclonal format increases tolerance for minor epitope variation across orthologues and is well-suited to Western blot detection of the full-length approximately 140 kDa glycoprotein, while the unconjugated preparation leaves open the choice of fluorophore or enzyme conjugate tailored to the downstream assay.

From Antibodies.com, each lot is quality-controlled and ships ready to use at working concentration for most Western blot protocols. Researchers studying mucosal immunology, transplant tolerance, autoimmune models, or myeloid cell differentiation in mouse and rat systems frequently choose this reagent, and the cross-reactivity to human CD101 extends the same antibody to clinical cohorts where paired donor material is available.