Anti-CCT5 Antibody

The CCT5 protein (Chaperonin Containing TCP1 Subunit 5, also called CCT-epsilon) is a subunit of the TRiC/CCT chaperonin complex, an eight-subunit ATP-dependent machine that assists the folding of cytoskeletal proteins such as actin and tubulin in the eukaryotic cytosol.

This rabbit polyclonal antibody from Antibodies.com recognizes CCT5 and is validated for Western blot and immunohistochemistry on samples from human, mouse, and rat origin. The unconjugated reagent ships as 100 µL of a 1 mg/mL stock in PBS supplemented with 0.1% sodium azide and 50% glycerol as a cryoprotectant.

For Western blot, separate samples by SDS-PAGE and detect with HRP- or fluorophore-conjugated secondary antibodies. For IHC, the antibody performs well on formalin-fixed paraffin-embedded tissue sections following heat-induced antigen retrieval. Studies on chaperonin biology, cytoskeletal protein folding, and the links between CCT dysfunction and hereditary sensory neuropathy rely on reagents like this to track CCT5 expression across disease and control tissues.

Store the vial at -20°C for long-term use; thaw on ice and avoid repeated freeze-thaw cycles to preserve binding activity. Short-term handling at 4°C is appropriate for working aliquots in routine use.