Anti-CCNF Antibody

The CCNF (Cyclin F) rabbit polyclonal antibody from Antibodies.com is an unconjugated reagent raised against the human Cyclin F protein, an F-box substrate adaptor for the SCF E3 ubiquitin ligase complex that controls genome stability, centrosome duplication, and dNTP pool balance during S-phase. Validated for Western blot (WB) and immunohistochemistry (IHC) on human samples, the polyclonal antibody is supplied as a 100 µl aliquot of rabbit host serum, suitable for both denaturing protein gels and formalin-fixed paraffin-embedded tissue section staining. Investigators studying familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia use this reagent to characterize CCNF missense mutations that disrupt ubiquitylation of substrates such as TDP-43, while cell cycle and DNA repair laboratories apply it to follow Cyclin F-mediated degradation of CP110 and RRM2, two key cell cycle regulators targeted for proteasomal turnover. The reagent recognizes the native human CCNF protein in cell lysates and tissue sections, supporting work on ubiquitin-proteasome pathway function, post-mitotic neuron maintenance, and tumor suppressor mechanisms in which Cyclin F activity has been implicated.