Anti-CCNE1 (phospho T395) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically detects cyclin E1 (CCNE1) when phosphorylated at threonine 395, a modification associated with CDK2 activity during the G1/S cell cycle transition. By selectively binding the phospho-T395 form, the reagent enables researchers to distinguish the kinase-engaged pool of CCNE1 from total protein levels in western blot lysates, supporting studies of DNA replication initiation, cell cycle re-entry, and oncogenic signaling in tumor models with elevated cyclin E activity.

Supplied as a 100 µl vial of unconjugated rabbit IgG, validated for western blot on human samples, this phospho-specific antibody is suited to work on cell lines and tumor-derived material where CCNE1 dysregulation has been documented, including breast, ovarian, and endometrial cancer systems.

For storage, aliquot the antibody and store at -20 °C, avoiding repeated freeze-thaw cycles to preserve phospho-epitope recognition. The unconjugated format offers flexibility for in-house conjugation if a custom detection label is required. Intended for research use only.