Anti-CCNE1 (phospho T395) Antibody

Produced by Antibodies.com within their Primary Antibodies range, this Rabbit polyclonal IgG antibody recognises Cyclin E1 (CCNE1) when phosphorylated at threonine 395. It is supplied unconjugated as a liquid preparation that has been affinity purified.

The antibody is recommended for Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF) applications and has been validated against Human, Mouse and Rat samples.

It was raised against a synthetic phosphorylated peptide spanning the T395 region of human Cyclin E1 (NCBI accession NP_001229.1), and recognises a protein that migrates at 55 kDa. The formulation is Phosphate Buffered Saline at pH 7.3, containing 50% glycerol and 0.02% sodium azide. Vials ship at 4°C and should be aliquoted for storage at -20°C, avoiding freeze/thaw cycles. Suggested working dilutions are 1:500–1:2,000 for WB and 1:50–1:200 for ICC/IF.