Anti-CCNE1 Antibody

This rabbit polyclonal antibody targets cyclin E1 (CCNE1), the regulatory partner of cyclin-dependent kinase 2 that drives the G1/S phase transition and commits cells to DNA replication. Raised in rabbit hosts and supplied at 100 µl as an unconjugated IgG preparation, it is validated for western blot, immunohistochemistry, and flow cytometry workflows on human samples. Antibodies.com provides this reagent as a primary antibody for cell cycle, oncology, and proliferation research, supporting studies of tumor biology where Cyclin E1 overexpression is being investigated as a biomarker. The polyclonal format maximizes epitope coverage across the full-length cyclin E1 protein, while the unconjugated presentation allows researchers to pair the antibody with their own secondary detection reagents and choice of fluorophore or enzyme label. Used in western blot, the reagent resolves the cyclin band in lysates from proliferating cells; in IHC, it highlights nuclear staining patterns consistent with active cell cycle entry; and in flow cytometry, it enables quantitative assessment of Cyclin E1 expression across cell populations. Refrigerate the vial upon arrival and aliquot before freezing for long-term storage, avoiding repeated freeze-thaw cycles to preserve binding activity. The human reactivity profile makes this antibody appropriate for translational studies linking CCNE1 amplification and overexpression to cancer prognosis, while polyclonal recognition of multiple epitopes supports reproducible detection across experimental conditions.