Anti-CCND1 (phospho T286) Antibody

Phosphorylation of cyclin D1 at threonine 286 is a key regulatory event that triggers nuclear export and ubiquitin-dependent proteolysis of this G1/S cell-cycle driver. The rabbit polyclonal IgG reagent from Antibodies.com is raised against this specific phospho-epitope and selectively recognizes the phosphorylated form of human CCND1, enabling researchers to track the post-translational turnover signal independently from total cyclin D1 levels.

Supplied as 200 µl of unconjugated antiserum in a vial, the antibody is validated for western blot workflows and performs reliably against denatured, reduced human protein lysates. Its polyclonal format broadens epitope coverage around the phospho-T286 site, an advantage when probing samples where the modification may be sub-stoichiometric or transient. Investigators studying G1 arrest, oncogenic signaling, and DNA damage responses can use this reagent to monitor the phosphorylation status of cyclin D1 across many cell lines and tumor-derived samples.

Storage: aliquot and store at -20 °C; avoid repeated freeze-thaw cycles to preserve phospho-specific reactivity. The host species is rabbit, the clonality is polyclonal, and the isotype is IgG. Reactivity has been confirmed for human CCND1.