Anti-CCNB1 (phospho S147) Antibody

Monitor phosphorylation of cyclin B1 at the regulatory serine 147 mark with this rabbit polyclonal IgG antibody from Antibodies.com, supplied as a 50 µL unconjugated preparation.

Cyclin B1 partners with CDK1 to form the maturation-promoting factor that drives the G2/M transition, and phosphorylation at S147 sits within a cluster of mitotic regulatory modifications influenced by upstream kinases such as PLK1. The antibody recognises the human phospho-S147 epitope and is validated for western blotting, immunohistochemistry, and immunofluorescence on cultured cells and paraffin or cryosectioned tissues after standard antigen retrieval.

In western blot, the reagent resolves a band near the expected cyclin B1 molecular weight and is particularly useful when comparing synchronised populations across cell-cycle stages. For immunostaining, it supports both chromogenic IHC and fluorescence-based imaging in tumour and tissue sections where mitotic index or checkpoint status is being investigated.

For handling, prepare single-use aliquots on first thaw and store the vial at -20 °C; avoid repeated freeze-thaw cycles to preserve phosphoepitope binding, and keep working dilutions refrigerated during short experiments. The reagent is buffered with glycerol as a cryoprotectant and can be diluted directly into standard blocking and washing solutions used for immunoblotting and immunohistochemistry. Materials are intended for research use only and have not been validated for clinical or diagnostic applications.