Anti-CCNB1 Antibody

This rabbit polyclonal IgG antibody from Antibodies.com targets CCNB1 (Cyclin B1), a regulatory cyclin protein that partners with CDK1 to drive the G2/M transition of the cell division cycle. The unconjugated reagent is supplied as a 200 µl liquid aliquot and is validated for western blot detection of human Cyclin B1.

Cyclin B1 abundance rises during S phase, peaks at the G2/M boundary, and is rapidly degraded after mitotic exit via the APC/C ubiquitin ligase, so this reagent is commonly used to monitor mitotic entry, checkpoint status, and proliferative state in cell lysates. CCNB1 overexpression and mislocalization have been reported in a range of tumor types, supporting research into cell-cycle dysregulation, mitosis, and oncogenesis.

As a polyclonal reagent raised in rabbit, the antibody offers multi-epitope recognition of Cyclin B1, which can improve signal robustness across denatured lysate preparations. For western blot, the antibody is incubated with whole-cell or tissue extracts separated by SDS-PAGE, with the IgG isotype compatible with standard anti-rabbit secondary reagents.

Store refrigerated in the supplied vial; avoid repeated freeze-thaw cycles by preparing working aliquots before long-term storage. For research use only.