Anti-Catenin-β Antibody

Antibodies.com supplies this rabbit polyclonal reagent against β-catenin, the ~88 kDa dual-function protein encoded by CTNNB1 that bridges classical cadherins to the actin cytoskeleton at adherens junctions and serves as the transcriptional co-activator of canonical Wnt signaling. Researchers use the antibody to track the cadherin-bound membrane pool, the cytoplasmic pool that is normally earmarked for destruction by the APC/Axin/GSK-3β complex, and the nuclear pool that partners with TCF/LEF factors to drive Wnt target genes such as AXIN2, c-MYC, and Cyclin D1.

Validated for Western blot and ELISA on human, mouse, and rat samples, the unconjugated polyclonal is supplied as a 100 µl liquid aliquot that fits standard immunoblotting of whole-cell lysates, recombinant standards, and fractionated nuclear extracts. Common applications include profiling Wnt pathway activation in colorectal and hepatocellular carcinoma models carrying stabilizing CTNNB1 mutations, monitoring β-catenin redistribution during epithelial–mesenchymal transition, following β-catenin dynamics in intestinal crypt and embryonic stem cell systems, and characterizing synaptic cadherin–catenin complexes in neuronal cultures.

For Western blot, the antibody detects the full-length ~88 kDa band in lysates separated by SDS-PAGE. The product ships as a single 100 µl vial ready for dilution into blocking or primary incubation buffer, and should be aliquoted on receipt to limit freeze-thaw cycles, refrigerated for routine use, and frozen for long-term storage. Intended for research use only in laboratory studies of cell adhesion, Wnt biology, and oncogenic signaling.