Anti-Catenin-α E / N Antibody

This rabbit polyclonal reagent from Antibodies.com targets the N-terminal region of Catenin-α E (alpha-E-catenin, also referred to as CTNNA1), a key component of the cadherin–catenin complex that anchors classical cadherins to the actin cytoskeleton at epithelial adherens junctions. Supplied as an unconjugated 100 µl vial, it supports Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows on Human, Mouse, and Rat samples.

Catenin-α E also interfaces with the Wnt/β-catenin signaling axis and contributes to cytoskeletal remodeling during morphogenesis and tissue homeostasis, making this antibody useful for studies of epithelial polarity, cancer cell invasion, intestinal barrier biology, and embryonic development. The polyclonal host response provides multi-epitope recognition, which can improve detection of partially degraded target protein or boost signal in low-abundance samples. For best stability, store the antibody refrigerated in working aliquots to limit repeated freeze-thaw cycles of the stock solution.

Suggested starting ranges for this reagent are 1:500 to 1:2000 for Western blot and 1:50 to 1:200 for immunohistochemistry, although optimal dilution should be determined empirically for each sample preparation and detection method. Because the immunogen maps to the N-terminus, the antibody recognizes full-length alpha-E-catenin and is not directed at C-terminal truncations or short isoforms that lack this region. Reactivity has been validated specifically for Human, Mouse, and Rat species, and the reagent is intended for research use only.