Anti-Catenin-β (phospho-T41/S45) Antibody

Phosphorylation at threonine 41 and serine 45 is a key regulatory event in β-catenin turnover, priming the protein for subsequent GSK-3β phosphorylation and proteasomal degradation via the canonical Wnt signaling pathway. This rabbit polyclonal antibody from Antibodies.com specifically recognizes the dual phospho-T41/S45 epitope on Catenin-β, enabling researchers to monitor the priming step of β-catenin degradation in tissue sections. The reagent is validated for immunohistochemistry on human, mouse, and rat samples and is supplied as 100 µl of a 1 mg/ml solution in phosphate buffered saline (pH 7.2) containing 0.05% sodium azide as a preservative. Investigators working on Wnt-driven colorectal cancer, hepatoblastoma, and developmental signaling can use this antibody to distinguish primed β-catenin from the signaling-competent, unphosphorylated pool in fixed tissue. The unconjugated format supports pairing with HRP-conjugated or fluorophore-conjugated secondary antibodies for chromogenic or fluorescence-based detection in standard IHC workflows. Refrigerate on receipt; protect from light; avoid repeated freeze-thaw cycles of the antibody solution to preserve activity.