Anti-Catenin-β (G650) Antibody

Beta-catenin (CTNNB1) functions as a scaffolding component of the cadherin adhesion complex at the plasma membrane and as a nuclear effector of canonical Wnt signaling, making it a widely studied target across oncology, developmental biology, and stem cell research. This polyclonal reagent from Antibodies.com is raised in rabbit and specifically recognizes the G650 epitope region of beta-catenin, supporting detection of endogenous protein across multiple experimental systems.

Validated for Western blot, immunohistochemistry, and immunofluorescence workflows, the antibody reacts with samples from human, mouse, and rat origins. It is supplied unconjugated at 1 mg/ml in phosphate buffered saline (pH 7.2) with 0.05% sodium azide as a preservative, packaged as 50 µl per vial. The unconjugated format allows researchers to conjugate the antibody with their preferred reporter — HRP, biotin, Alexa Fluor dyes, or other labels — depending on assay design.

For western blotting, dilute the antibody between 1:500 and 1:2000 in blocking buffer; for IHC and IF on fixed sections, working concentrations in the 1:50 to 1:200 range are common starting points. The polyclonal nature of the reagent provides multiple epitope recognition sites, often yielding stronger signals for low-abundance targets than monoclonal alternatives. Aliquot upon arrival, keep refrigerated, and avoid repeated freeze-thaw cycles to preserve activity over the working shelf life of the reagent.