Anti-Catenin-β Antibody

Beta-catenin (encoded by CTNNB1) is a dual-function protein central to canonical Wnt signaling and to E-cadherin-linked adhesion at the adherens junction. Aberrant stabilization and nuclear translocation of beta-catenin drive oncogenic programs in colorectal carcinoma, hepatocellular carcinoma, and other epithelial cancers, which is why rigorously validated antibodies against this target are foundational across cancer biology, developmental biology, and epithelial cell research.

This recombinant rabbit monoclonal reagent from Antibodies.com is supplied as 100 µl of unconjugated antibody in 1× TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, packaged in a single vial. It has been validated for western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation workflows, and it reacts with human, mouse, and rat samples, supporting both denaturing IP pull-downs and fixed-cell or paraffin-section staining protocols.

Typical applications include tracking Wnt-driven nuclear accumulation in stimulated cell lines, profiling membrane-associated beta-catenin pools in tissue architecture studies, and immunoprecipitating endogenous complexes from lysates for downstream mass spectrometry or co-immunoprecipitation partner analysis.