Anti-Caspase 9 (Tyr153) Antibody

A rabbit polyclonal antibody from Antibodies.com specifically recognizes caspase 9 only when phosphorylated at tyrosine 153, a regulatory site implicated in modulating the intrinsic mitochondrial apoptosis cascade. The immunogen-derived reagent is supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) containing 15 mM sodium azide at approximately pH 7.2, and ships as a 50 µl vial sized for routine western blot workflows. Validated reactivity spans human, mouse, and rat samples, enabling cross-species comparison of phosphorylated caspase 9 signaling in cell death studies, neurobiology, and oncology research. For handling, refrigerate the vial upon arrival and aliquot into smaller working volumes to limit freeze-thaw cycles; sodium azide is included as a preservative. Dilutions should be optimized empirically for each lysate preparation, and detection is generally performed using HRP-conjugated secondary reagents with standard chemiluminescent readout.