Anti-Caspase 9 (Tyr153) Antibody

This polyclonal antibody reagent from Antibodies.com specifically targets Caspase 9 at the Tyr153 residue, a post-translational modification site implicated in the regulation of intrinsic apoptotic signaling. Raised in rabbit, the antibody is supplied as 100 µl of unconjugated antibody at a concentration of 1 mg/ml in phosphate buffered saline (PBS) containing 15 mM sodium azide, at approximately pH 7.2.

Validated for Western blot (WB) workflows, the reagent detects endogenous Caspase 9 in human, mouse, and rat samples, supporting comparative studies across model organisms. Its specificity for the Tyr153 epitope makes it valuable for researchers investigating phosphorylation-dependent control of initiator caspase activity under conditions such as cellular stress, mitochondrial damage, or chemotherapeutic challenge.

Storage guidance: refrigerate the vial at 4°C for active use. For extended storage, freeze aliquots at -20°C to preserve antibody integrity and minimize freeze-thaw cycles. The 15 mM sodium azide serves as a microbial preservative; the additive is compatible with standard immunoblocking and incubation steps in membrane-based Western blot detection.

Format: supplied as a 100 µl liquid aliquot in a single vial, used as the primary antibody in chemiluminescent Western blot detection of Caspase 9 from cell lysates and tissue homogenates.