Anti-Caspase-9 Antibody

Caspase-9 is the apical initiator caspase of the intrinsic apoptosis pathway, recruited to the Apaf-1 apoptosome complex where cytochrome c-driven dATP binding triggers its dimerization and autocatalytic activation. Once processed, it cleaves downstream executioner caspases that dismantle the cell during programmed cell death.

The unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of antiserum, validated for western blot and ELISA workflows against human and non-human primate samples. As a cysteine protease central to mitochondrial-dependent apoptosis, caspase-9 is a routine readout in cancer biology, neurodegeneration studies, and cardiotoxicity screens, where collapse of mitochondrial outer membrane potential marks the commitment point to programmed cell death. The polyclonal format supports recognition of multiple epitopes across the full-length protein, enabling detection of both the inactive zymogen and its activated cleavage fragments during apoptosis induction. Each vial is intended for research use only and is compatible with standard immunoblotting buffers, HRP- or IRDye-conjugated secondary antibodies, and chemiluminescent or near-infrared detection platforms.