Anti-Caspase-9 Antibody

Caspase-9 is a cysteine-aspartic protease that serves as a critical initiator caspase in the mitochondrial (intrinsic) apoptotic pathway. Upon assembly of the apoptosome complex with Apaf-1 and cytochrome c, the inactive pro-Caspase-9 zymogen is cleaved and activated, triggering a downstream caspase cascade that drives programmed cell death. Researchers studying neurodegeneration, cancer cell death pathways, ischemic injury, and developmental biology frequently probe Caspase-9 expression and cleavage status to evaluate apoptotic signaling in their experimental models.

This unconjugated rabbit polyclonal antibody from Antibodies.com is supplied as 100 µl of liquid and is validated for use in western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. It recognizes endogenous Caspase-9 across human, mouse, and rat samples, enabling comparative studies in cell lines, primary tissue sections, and animal model systems. The reagent supports detection of both the full-length proform (~46 kDa) and the cleaved active fragment, making it useful for monitoring activation dynamics in response to pro-apoptotic stimuli such as staurosporine, UV irradiation, or chemotherapeutic agents.

For best results, aliquot upon arrival and store at -20°C to preserve reactivity across multiple freeze-thaw cycles. Working dilutions should be optimized empirically for each application, with typical starting ranges of 1:500 to 1:2000 for western blotting and 1:50 to 1:200 for immunohistochemistry on fixed sections.