Anti-Caspase-8 Antibody

Caspase-8 is a cysteine-aspartic protease that initiates the extrinsic apoptosis pathway through death receptor signaling, and this Antibodies.com primary antibody enables direct interrogation of the protein in whole-cell lysates and fixed tissue sections. The reagent is a mouse monoclonal, unconjugated antibody supplied as 100 µl of liquid stock validated for western blot and immunohistochemistry workflows. Reactivity is confirmed against Human, Mouse, and Rat samples, supporting comparative apoptosis research across standard laboratory model systems. The unconjugated format permits flexible pairing with HRP-, biotin-, or fluorophore-conjugated secondary antibodies depending on the detection chemistry required.

Typical downstream uses include tracking of full-length procaspase (~55 kDa) alongside its cleaved p43/p41 and p18 subunits, investigation of death receptor signaling in cancer cell lines, and characterization of apoptotic versus necroptotic responses in tissue specimens. To preserve activity, store refrigerated and prepare small aliquots to limit freeze-thaw cycles when the antibody is used intermittently.