Anti-caspase-8 Antibody

Caspase-8 is a cysteine-aspartic protease that acts as the apical initiator caspase of the extrinsic apoptosis pathway, transducing death signals from cell surface Fas and TRAIL receptors through FADD-mediated cleavage of downstream effector caspases. This unconjugated rabbit polyclonal reagent from Antibodies.com recognizes the full-length ~55 kDa caspase-8 proenzyme as well as its processed cleavage fragments generated during Death-Inducing Signaling Complex (DISC) assembly, supporting western blot detection of both zymogen and activated forms in human, mouse, and rat samples.

The 100 µl aliquot is validated for immunofluorescence and immunohistochemistry workflows, enabling visualization of receptor-proximal caspase activation at tissue and subcellular levels where the extrinsic death pathway is under investigation. Researchers studying necroptosis regulation, ripoptosome signaling, or chemoresistance mechanisms use this reagent to probe how caspase-8 catalytic activity is balanced against its scaffold function within the ripoptosome complex.