Anti-Caspase-6 p18 (D179) Antibody

This rabbit polyclonal antibody from Antibodies.com targets the cleaved p18 subunit of human Caspase-6, a cysteine aspartyl protease central to apoptotic cell death programs. Generated against a peptide region surrounding the Asp179 cleavage site, the reagent preferentially recognizes the active large subunit produced when pro-caspase-6 is processed during apoptosis induction.

Validated for Western blot and immunohistochemistry workflows, the antibody cross-reacts with mouse and rat orthologues in addition to human samples, supporting comparative studies across cell culture and fixed tissue. It is supplied as 100 µl of liquid at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, and is unconjugated for flexible pairing with HRP-, biotin-, or fluorophore-conjugated secondary reagents.

Typical research applications include profiling executioner caspase activation in neuronal cultures and brain sections relevant to neurodegenerative disease, characterizing chemotherapy-induced apoptosis in tumor cell lines and xenograft tissue, and confirming pathway engagement in developmental cell death models. Storage at -20 °C in working aliquots helps preserve activity and limits repeated freeze-thaw cycles of the stock reagent.