Anti-Caspase-3 p17 (D175) Antibody

A polyclonal antibody from Antibodies.com targeting the cleaved p17 subunit of caspase-3, raised in rabbit and supplied at 1 mg/mL in PBS containing 0.1% sodium azide and 50% glycerol. The reagent specifically recognizes the p17 fragment generated when full-length caspase-3 is processed at the D175 (aspartate 175) site during apoptotic signaling cascades, providing a marker for the catalytically active protease rather than the inactive zymogen form. Validated for western blot, the antibody detects a band of approximately 17 kDa on reducing SDS-PAGE gels and has been characterized for reactivity with human, mouse, and rat samples, supporting cross-species apoptosis studies in cell lysate and tissue homogenate workflows. Each vial contains 100 µL of ready-to-use stock, drawn from a single immunogen lot to maintain lot-to-lot consistency across multiple experimental runs. Aliquot the stock into single-use portions upon arrival and store frozen at -20 degrees C to limit freeze-thaw cycles; keep the working solution refrigerated and protect from light during handling. Researchers investigating programmed cell death, mitochondrial pathway engagement, or downstream executioner caspase signaling rely on this reagent to distinguish cleaved protease activity following chemotherapeutic challenge, growth factor withdrawal, or receptor-mediated apoptosis induction in vitro and in vivo.