Anti-Caspase 10 Antibody

Caspase 10 Polyclonal Antibody for Apoptosis Research

Produced in rabbit and affinity-purified against the full-length human Caspase 10 protein, this unconjugated polyclonal antibody from Antibodies.com is validated for Western blot, immunohistochemistry, and immunofluorescence workflows in human samples. Caspase 10 is an initiator caspase in the extrinsic apoptosis cascade, recruited to the death-inducing signaling complex (DISC) following Fas and TNFR family receptor activation, where it undergoes proximity-induced autoprocessing and propagates the apoptotic signal to downstream effector caspases such as Caspase 3 and Caspase 7.

The reagent is supplied as a 100 µl aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, supporting convenient long-term storage and minimizing freeze-thaw degradation of the antibody. Sodium azide acts as a microbial preservative; researchers performing live-cell applications should remove it by dialysis or buffer exchange prior to use.

Common research uses include studies of Fas-mediated and TRAIL-mediated programmed cell death, autoimmune lymphoproliferative syndrome type II linked to CASP10 mutations, and cancer cell death signaling pathways. The polyclonal format offers epitope diversity that helps preserve recognition when a particular cleavage site or post-translational modification is masked in a given sample.