Anti-CASP3 Antibody

Produced by Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against a recombinant human CASP3 immunogen and is supplied at 1.0 mg/mL in a 50 µL volume of phosphate-buffered saline (pH 7.4, 150 mM NaCl) containing 0.02% sodium azide and 50% glycerol. The unconjugated antibody recognizes the endogenous total CASP3 (caspase-3) protein and has been validated for western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF) and immunohistochemistry (IHC). Cross-reactivity has been confirmed in human, mouse and rat samples, making the reagent suitable for apoptosis-related studies across these three species. For routine handling, the product is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, with repeated freeze/thaw cycles avoided to preserve activity. Each lot is purified by antigen-affinity chromatography to reduce nonspecific binding, supporting clean detection of full-length and processed caspase-3 in cell lysates and tissue sections. Intended for research use only, this primary antibody from Antibodies.com provides a reliable tool for laboratories investigating programmed cell death, caspase activation pathways and related signalling research.