Anti-CASP3 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against CASP3 (caspase-3), the cysteine-aspartate protease that acts as a central executioner of apoptotic signaling in vertebrate cells. Supplied in a 100 µl volume, the reagent is validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human, mouse, and rat specimens.

Caspase-3 is produced as an inactive ~35 kDa zymogen and is cleaved at Asp175 during apoptosis, generating the p17 and p12 fragments that assemble into the active heterotetramer. Researchers rely on the polyclonal format to detect both the full-length pro-enzyme and the cleaved subunits in cell lysates and tissue sections, enabling readouts of activation state across cancer, neurodegeneration, and developmental biology models.