Anti-CASP3 Antibody

This primary antibody from Antibodies.com is a rabbit polyclonal reagent raised against CASP3 (Caspase-3), the cysteine-aspartic protease that serves as the central executioner of apoptotic cell death. The unconjugated antibody is supplied in a 100 µL volume and is validated for Western blot and immunohistochemistry workflows, with confirmed reactivity against human, mouse, and rat samples.

Caspase-3 is synthesized as an inactive 35 kDa proenzyme that is cleaved at Asp175 during apoptotic signaling to generate the p17 and p12 fragments that assemble into the active heterotetramer. Because both the full-length precursor and the cleaved subunits can be detected with appropriate sample preparation, the antibody is commonly used to monitor activation status alongside PARP cleavage and other downstream apoptotic markers in cell lysate and fixed-tissue experiments.

The polyclonal format provides multi-epitope recognition of the target, which is helpful when probing conserved regions across species or when working with denatured lysates. Investigators studying neurodegeneration, cancer cell death, ischemic injury, and developmental biology routinely rely on this reagent to quantify apoptotic indices. Aliquot upon receipt and store refrigerated to preserve performance across repeated freeze-thaw cycles.