Anti-CASP3 Antibody

This rabbit polyclonal antibody targets Caspase-3 and is offered by Antibodies.com as a primary antibody reagent. It is unconjugated, of the IgG isotype, and reacts with human, mouse, and rat samples. The antibody has been validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF / ICC/IF) applications, and is reported as KO validated.

The immunogen is a recombinant fusion protein containing a sequence corresponding to amino acids 29-175 of human Caspase-3 (NP_004337.2). The product is supplied as a liquid in Phosphate Buffered Saline, pH 7.3, containing 50% Glycerol and 0.01% Thiomersal, and has been affinity purified. Reported molecular weight targets are 32 kDa and 17 kDa, with concentration described as lot specific.

Recommended dilutions are WB 1:500-1:1,000, IHC 1:50-1:200, and ICC/IF 1:50-1:200. The antibody is shipped at 4°C; upon delivery it should be aliquoted and stored at -20°C, avoiding freeze/thaw cycles.