Anti-CASP3 (active) Antibody

Raised in rabbit and affinity purified, this unconjugated polyclonal antibody from Antibodies.com selectively recognizes the cleaved, catalytically active form of caspase-3 rather than the full-length zymogen. During apoptosis the inactive pro-caspase-3 is processed into p17 and p12 subunits, and the reagent is validated to detect these fragments in western blot and immunohistochemistry workflows on human, mouse, and rat samples.

Typical applications include characterization of apoptotic signaling in cell culture models and in tissue sections, where active caspase-3 marks cells committed to programmed cell death. The 100 µl vial is supplied at a concentration suitable for direct use or further dilution depending on antigen abundance and detection chemistry, and the unconjugated format gives flexibility to pair the antibody with a secondary reagent of the researcher's choice.

For handling, store frozen at -20 °C in working aliquots to limit freeze-thaw cycles and protect the protein from degradation; brief cold storage is acceptable during active experimental use, and the reagent should be protected from light. Each lot is quality controlled by Antibodies.com for reactivity across the three listed species and is intended for research use only.