Anti-CARD8 Antibody

This polyclonal primary antibody from Antibodies.com targets CARD8 (Caspase Activation and Recruitment Domain Family Member 8), a CARD-containing scaffolding protein best known for its role as a negative regulator of the NLRP3 inflammasome and for modulating caspase-1-mediated interleukin-1β maturation. Raised in rabbit, the reagent is unconjugated and supplied as 100 µl of liquid, validated for western blot (WB) and immunohistochemistry (IHC) workflows on human, mouse, and rat samples.

CARD8 is expressed across epithelial and immune cell lineages and has been investigated in the context of inflammatory signaling, apoptosis regulation, and several cancer cell lines, where altered CARD8 levels have been linked to proliferation and pyroptosis phenotypes. Researchers working on inflammasome biology, NF-κB pathway crosstalk, or tumor immunology will find this reagent useful for probing endogenous CARD8 expression in tissue sections and lysates.

For best results, aliquot the antibody upon arrival, refrigerate working stocks, and avoid repeated freeze-thaw cycles to preserve activity. Each lot is validated by Antibodies.com for use in WB and IHC applications, and the cross-reactivity profile covers the three listed species, providing a flexible tool for comparative studies.