Anti-CAP2 Antibody

CAP2 (Cyclase-Associated Actin Cytoskeleton Regulatory Protein 2) is a 477-residue actin-binding protein that controls monomer sequestration and filament turnover, with a calculated molecular weight of approximately 53 kDa. The protein is concentrated in brain, heart, skeletal muscle, and skin, where it localizes to the M-line of differentiated myotubes and to lamellipodial actin networks. Loss-of-function mutations in CAP2 cause a recessive dilated-cardiomyopathy syndrome with supraventricular tachycardia, and overexpression has been documented in hepatocellular carcinoma, breast cancer, and malignant melanoma, where it supports lamellipodium formation and cell motility. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com was raised against the human CAP2 target and detects endogenous protein in human, mouse, and rat samples by western blot and immunohistochemistry. The 200 µL vial is supplied in PBS buffer with 0.05% sodium azide and 50% glycerol; aliquot on receipt and store at -20 °C, avoiding repeated freeze-thaw cycles. In western blot, the antibody recognizes a band near the predicted 53 kDa size in lysates such as HepG2, HeLa, and mouse skeletal-muscle tissue. For immunohistochemistry on paraffin sections, working dilutions of approximately 1:50 to 1:200 are commonly used, with citrate or TE pH 9.0 antigen retrieval giving robust nuclear and cytoplasmic staining in skeletal-muscle and tumor sections. The unconjugated format lets laboratories pair the antibody with their own HRP- or fluorophore-labeled secondary reagents, which is useful when multiplexing CAP2 with cytoskeletal markers such as α-cardiac actin, tropomyosin, or CAP1 in studies of cardiomyocyte maturation, sarcomere remodeling, and actin pointed-end dynamics.